Salt Tolerance Enhancement of Liquid Chromatography-Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Using Matrix Additive Methylenediphosphonic Acid
نویسندگان
چکیده
منابع مشابه
An overview of matrix effects in liquid chromatography-mass spectrometry.
Matrix-dependent signal suppression or enhancement represents a major drawback in quantitative analysis with liquid chromatography coupled to atmospheric pressure ionization mass spectrometry (LC-API-MS). Because matrix effects (ME) might exert a detrimental impact on important method parameters (limit of detection, limit of quantification, linearity, accuracy, and precision), they have to be t...
متن کاملOvercoming matrix effects in liquid chromatography-mass spectrometry.
A major limitation in quantitative analysis with electrospray ionization mass spectrometry (ESI-MS) is represented by the so-called matrix effects in which the matrix coextracted with the analytes can alter the signal response, causing either suppression or enhancement, resulting in poor analytical accuracy, linearity, and reproducibility. In the direct electron ionization liquid chromatography...
متن کاملApplication of Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry
The clarification of metabolic dynamics in lesion areas is important. Many approaches, such as high performance liquid chromatography mass spectrometry, gas chromatography mass spectrometry, immunohistochemistry, are used to define disease-related abnormalities. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is attracting attention as a new valuable tool. MALD...
متن کاملMatrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry
A new matrix assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) method to spatially profile the location and distribution of multiple N-linked glycan species in tissues is described. Application of an endoglycosidase, peptide N-glycosidase F (PNGaseF), directly on tissues followed by incubation releases N-linked glycan species amenable to detection by MALDI-IMS. The meth...
متن کاملHigh-accuracy molecular mass determination of proteins using matrix-assisted laser desorption mass spectrometry.
A method for obtaining protein molecular masses with an accuracy of approximately +/- 0.01% by matrix-assisted laser desorption using an internal calibrant is described. The technique allows accurate mass determinations of protein sample sizes as small as 1 pmol. High concentrations of organic and inorganic contaminants (e.g. 1 M urea) do not strongly affect either the signal intensity or the m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Mass Spectrometry
سال: 2014
ISSN: 2187-137X
DOI: 10.5702/massspectrometry.a0031